Although I don’t really support doing a brute-force approach doing manual variants review, if you only have some of your top signal that you would like to confirm for further wet-lab experiment validation, IGV might still proves helpful.
This review by Robinson, et al from a group at UCSD shed some lights and detail into how you can do the manual review in IGV: http://cancerres.aacrjournals.org/content/77/21/e31
I also found the IGV manual describing all the options in the preference menu to be quite useful: https://software.broadinstitute.org/software/igv/Preferences
Aug 09 (GenomeWeb) Geisinger team announced their approach to return secondary finding the genomic tests to participants.
Read the full article on AJHG at https://www.cell.com/ajhg/fulltext/S0002-9297(18)30236-2
As a bioinfomatician, one might get into the situation that requires you to run that software in certain environment again and again, unfortunately, on various computers.
The good news is there’s this thing called “Docker”, a container technology that might allow you to do so easier. If you are a fan of “Singularity”, you already know what I’m talking about here.
Without further ado, here’s what you will need:
- You’ll need to install Docker on your computer. Also, get a docker hub account. You’ll need this to deposit your docker image.
- Get the source code or the pre-compiled software that you want to run
- Create a new working directory to store your
Dockerfile (see below)
- The most important step, which will determine how complicate your life will be authoring a
Dockerfile is how you choose your base image.
- Assuming you are trying to run java application, your best bet for the base image would be this image family
Consider the following example (stolen from Broad’s Institute tutorial for FireCloud https://software.broadinstitute.org/firecloud/documentation/article?id=9453)
Continue reading “Quick start on “Creating a new docker container””
Sanny wrote on https://superuser.com/questions/1075576/ms-word-for-mac-latest-version-resetting-normal-dotm
From this thread in Apple Exchange, the folder’s location has changed for Office for Mac 2016. It’s now located in
~/Library/Group Containers/UBF8T346G9.Office/User Content/Templates
For Office for Mac 2011 or if you upgraded from this edition to 2016, browse to this location
~/Library/Application Support/Microsoft/Office/User Templates/My Templates
In Windows, I can delete the file
normal.dotm to reset it. So you should try doing so in your Mac. You need first to close Word before deleting.
Normally, if you pinned an app to your “Dock” [the icons at the bottom of the screen], you can simply right-click the app icon and choose > Options > Open at log-in to change this setting.
However, Google Chrome, for some reasons, at the moment, does not have this option checked, but still loaded automatically at log-in.
To disable this, on MacOS Seirra, (also true from Lion on wards), Check the System Preferences > Users & Groups > [your username] > Login Items.
You might see “Google Chrome” in the list of Login Items. By removing it from the list, Chrome should not automatically be loaded anymore.
You may have a problem trying to forward X11 from your Linux server through a terminal in Windows. I use Bitvise as the ssh client as it also provides the interface for sftp to download/upload the files. Not that using command line scp or sftp is not efficient, but in Windows you may have to install those tools separately.
Since I was trying to avoid installing Cygwin initially, Cygwin terminal wasn’t what I initially consider. Therefore, I went ahead and install Bitvise ssh client (instead of putty), which actually turns out to be quite good. The only problem (at least now) is when trying to forward GUI from the server with X11 forwarding, it failed miserably.
- I’ve tried setting up bitvise in the X11 forward.
- Installed X11-server from Cygwin (finally T_T).
- But X11 forwarding still did not work. I got the error message below.
Failed to open channel for X11 forwarding from [::1]:47396 to 127.0.0.1:6000. Error connecting to X11 server: FlowSocketConnector: Failed to connect to target address. Windows error 10061: No connection could be made because the target machine actively refused it.
I kind of have an idea that this is something to do with Windows Firewall, which does not allow a specific port to listen to an incoming connection. But I haven’t quite figured out what to do. It is actually described on the bitvise page, but I guess I haven’t quite read the whole thing.
In short, after install cygwin X11 server, make sure to run the command below to fix the firewall permission.
C:\cygwin64\bin\XWin -listen tcp -multiwindow
Going through the AWS tutorial about creating File GateWay on my own is not exactly a walk in the park. Although I managed to install awscli and configured the basic requirement, there’s a missing gap that I haven’t quite figured out. This saves me lots of time trying to figure out how I can back up data already live on ZFS FreeNas to AmazonS3.
aws s3 sync /mnt/data/ s3://mybucket
What’s left to figure out next would be how to get all the files out when I need it, and how much it would cost, how to save those cost, etc. For the later part, I guess having AWS free credits does help a lot.
Take a look at the full guide following the link below.
ZFS is excellent at storing all your data safely but “there are still lots of potential ways for your data to die, and you still need to back up your pool. Period. PERIOD!” The process …
Source: Backing up FreeNAS to Amazon S3